I made this the last day of 2012 to encourage people to go on an adventure. My life took on a whole new adventure and went to the nations. Rarely am I am in USA anymore and when I am its still my home. Since March 17, 2011. My book was published in Dec 2016 called "Inspired by the Great Commission" Evangelist Angela Cummings. I have now been to 50 countries and showed my book to one of the designers of the Honda Fit in Tokyo, Japan. You can see the video if you are interested. God is amazing and His plan was perfect. Glory to God! Jesus was homeless too. Now, He is on Throne in heaven praying for us all.
“I think the recession changed the playing field for a lot of people,” notes Sofia Borges, an architect, trend consultant, and lecturer at the University of Southern California. “Job security, homeownership – a lot of that went out the window and never really returned. When a change like that happens, you have to change your ideas a little bit too.”
I am male 38 years old and I have lived in my 2005 Prius in Dallas,Texas for 9 months. I have chosen to adapt my life to living out of a car to see what life it might create. After getting rid of 85% of my stuff and buying a Dreamtime Therma-rest pad from REI and a piece of plywood to extend the backseat by 4 inches beyond the seats laying down, I am living in plushville. So far missing a internet connection in the car has been the biggest thing i would like. I am thinking the 3G iPad will be my next logical leap. I also realized that past 85 degrees sleeping in my car is unpleasant ( i am a bit of a princess and i prefer not to sleep with the car on except in extreme weather conditions). I need to procure a solar powered fan with a battery by next summer (note to self).

This post was inspired by my first experience with a police officer. I was parked in the Mockingbird DART station parking lot( I really like this spot for some reason). After having parked there for over 4 months I got a knock on my door. They told me to get out and hand them my license. They checked my record and asked me to move on. They asked me has anyone ever told you you can’t park here? I said no.

Yes, so long as you park your home-slash-car in an authorised area. Get yourself to a dedicated gypsy site or get permission from a private land owner and you’ll be fine, in theory. The sites we spoke to said they wouldn’t consider taking on a person living in a car (even a flash one) but you may have better luck, especially if you can convince the owner you’ll take them for a spin in your Ferrari-cum-bedroom every day. The question of whether sleeping in a car is preferable to sticking around at home is wholly subjective, but having looked into it, we’re erring on the side of bricks and mortar.
What an awesome blog. I am so glad that I came across it. I have been living in a friends apartment garage for free. But now her son and his girlfriend want to move up there so now I need to leave. I went through a bad custody battle and lost to an abusive ex. It is all about who has the most money. And it wasn’t me. So now I am in debt with to attorney fees (2 attorneys), and medical expenses. Even if you have insurance you have to sell your lung to pay for things. So now I have been trying to decide what do I do now. So I decided to live in my car. Right now I am packing. I am excited about the experience. I got a gym membership so I can shower, and I found a Loves truck stop where hopefully I can get a goods night rest. I dont have a PO Box yet because there is a waiting list. I didn’t realize there was such a high demand for PO boxes. I am trying to find a 24 hour storage close to my work so it won’t be out of the way when I need to go there. Same thing with my PO box. I live in Texas and I don’t have any family. Plus I was in foster care growing up. The closest thing I have to family is my co-workers. They are amazing! But I don’t want anybody to know.
Even when you have permission to park, some cities have specific laws against “vehicle vagrancy,” or people living in cars. The city council in Palo Alto, California, passed a law in August 2013 that makes living in a car illegal, citing safety concerns over too many vehicle dwellers in a community center parking lot. Violators could be charged with a misdemeanor and up to six months in jail and a $1,000 fine.

you can get an optima deep cycle battery, $200-$250. and this will give you all kinds of electrical power. like for your electric blanket all night long. connect it to the car with cables so that when the car is running, it is charging the optima battery. an optima yellow deep cycle battery or blue deep cycle battery will give you lots of electrical power. you can drain it to almost nothing lots of times and not ruin the battery. it does not put out fumes. only get an optima battery, others put out toxic fumes. they are more for boating or open air applications.

Yeah, of course I thought about those questions, but my answers were never good enough. My loved ones had their intentions in the right place, though; it’s true that the van dwelling lifestyle isn’t as easy as packing all your things in a vehicle and driving away. If you want to successfully prepare for living in your car, you need to understand the prep work involved and how your choices could impact your long-term comfort and safety.
Don’t forget to include a handheld vacuum cleaner that u can plug into the cigarette lighter. Don’t skimp on the motor size either get one with a strong motor for better cleaning. You’ll be surprised how the dirt you track into the car from your shoes will start making the car smelly musty. And make sure to pack separately last thing u want to do is break the plug end off. You get the idea.
Scared of impounded ‘homes’? Yes, that is a big deal. One tip is to go directly to the local police station and simply ask; do this before you’ve been living in your car too long, to avoid that whole “I’ll significantly reduce property values with my presence” look. On the road I met a fellow who would literally go town to town, seek out the police station and get a free night’s rest in an (unlocked) jail cell. Nice cot and nice company; although I’m not there yet.
I do have a judgmental comment but it is not at all directed at LIMH personally. Am I the only GRS reader who finds it totally pathetic that affordable housing in the U.S. is so difficult to come by? This is reminding me of a story in “Nickel and Dimed” by Barbara Ehrenreich. I don’t have the book in front of me, but I recall in one chapter how she’s working as a Merry Maid and finding out one co-worker is living out of his car and others are in weekly motels because there’s no affordable housing. One of the main points of the book is that it is nearly impossible to find adequate housing on a low salary. So much for the supposed greatest country in the world…
There are very few people who are able to live without money, and the percentage of car dwellers who have a sizable savings account is also limited. Most people who live out of a car will depend on having some type of income each month. Some of these car dwellers may receive social security, disability, or a pension. The rest probably need to work in order to earn money. Freelancers and those who are self-employed with businesses that can be operated from anywhere are able to boondock so long as they have access to needed services (electricity, Internet, etc.).

It has been very interesting since the last time I had posted on here. I have been here in Los Angeles for 3 months now, still living in my car. I had to move the car twice. I had to move the car from the recreational park that I use to park at because workers there caught on to what I was doing and decided to call the police. Believe me, it is not fun waking up to see a police officer with his gun pointed at you. After about nearly an hour dealing with them, they figured out that I was really a good guy trying to make it and told me it would be better to park in the parking lot at the VA hospital. I thought that was a good idea since I am an ex-marine. Well that lasted for a week until the federal police saw me and told me that I couldn’t sleep in my car on the lot and had to go. But, they did tell me of a very safe spot to park the car where it would not be bothered by LAPD or the Feds. It is near Brentwood, CA right outside the VA compound. Actually there are several other people living in their vehicles there as well. I actually got to meet a few of them and they are really cool people. I have been here ever since and do not have any trouble with police or parking enforcement. Also, there is a building right across the street where I can clean up and nobody even knows. I do still go to the UCLA campus to take showers, workout, or use their computers. Anyone can be a member at the gym on campus where it costs about $40 for the whole semester. I did get help from the state so that I could eat better. I was able to get the EBT card which has about $300 per month for food. Now, I will tell you, this really isn’t all that easy. You do, however, get use to it, but you just got to keep striving for better things. I am hoping that soon I will be able to get back into a house or apartment, however, I am very grateful that I have my car, because living on the street would probably be a bit too much for me.
But changing the alternator IS REAL MODIFICATION. and be aware that many places will not service your vehicle and you cannot afford that. I would prefer to not change the alternator on that ground, even if I could change my alternator, because when I need my car taken of, I really cannot have no where to go, or have a difficult time getting to a mechanic, because I have no other place other than my car.
Aiming at maximizing the chance for therapeutic benefit from the immunotherapy while minimizing the risk for life-threatening complications of CRS, researchers had outlined a proactive management strategy that incorporates a five-grading system and treatment algorithm designed to administer early immunosuppression for patients of high risk while avoiding unnecessary immunosuppression due to the potential risk of diminishing antitumor efficacy, based on their treating experiences.55 Besides, several investigators have developed CARs targeting fibroblast activation protein-α (FAP), which expressed on the surface of cancer-associated fibroblasts as a major component of the solid cancers stoma network, hoping to avoid the toxic effect.56 Paradoxically, some articles had reported that CAR-T cells targeting murine FAP could induce severe bone marrow toxicity while some other ones had demonstrated its antitumor benefits in the absence of toxicities in mice, indicating that more researches are needed to explain why some FAP-specific CARs induce toxicities and others do not.57,58
Funny story: After two-week dance festival in the Sierras, a couple of us decided to hang out. Four cars drew up to a open area in the woods…and everyone had their own setup & routine. Pros at the sleeping in cars/vans. It was hilarious. So different from the stay-in-motel/hotel crowd. At the time, I think I was the only homeless one. The rest all had apartments/houses.

Another option to consider- when you can't shower, use unscented baby wipes to clean up, or take a "bum shower" in a public restroom where you feel comfortable doing so. You also could learn local establishments that have single-person restrooms. Use them to washing you hair or face. Bring a towel to dry your head and the sink area, and be quick. Alternate between establishments.
Get some cashmere sweaters, second hand stores don’t even know what it is, 3 bucks for cashmere. and it is washable by hand. but the new fabrics for skiers are great too. A wool hat and some goose down comforters. I find that having a hat on at night and wool socks helps a lot. Goose down is vital wool blankets too. I put them into bags during the day so the dog stays off of them. He slept in the front seat. My cat had a pet box and went outside during the day. I parked at a place where she could go out and find me at night. (small fourplex with a large parking lot). My horse was bored and I had a tack room where I stored my things.It is easier to bored a horse in the city, then find an apartment.
e. A folding, hanging shower stall and a shower bag with nozzle makes for a hot shower even in freezing cold, and one can get dry and re-dressed before even feeling cold. For a floor, a baby inflatable 1-ring mini pool makes a perfect showerpan floor and warmly cleans your feet as you shower. Otherwise, use something else to keep your feet off the ground.
I’ve lived out of my car on and off in the past year, as a voluntary experiment with the vagabonding lifestyle. Large and mid-large SUV’s, when their seats are folded down, create a flat platform which functions as a good sleeping base. A mattress pad makes good cushion, and best of all, absolutely no one suspects an SUV parked overnight at Walmart to harbor a sleeping person, especially with the tinted rear windows. Space is rather limited though, and acrobatics are necessary to enter the sleeping section. If you are on an extreme budget, a rag and some soap in… Read more »
awesome site.. i have been staying in my minivan all summer. i am planning on putting in the winter also. i spent a winter 17 years ago living outside in alberta canada.. yes, it really does get to -85degrees celsius with the windchill. some tips that i used to use. if possible. work and stay awake and alert at night. sleep in the day. its warmer and safer. that of course is if you don’t have the protection of a shelter(vehicle).. i am in calgary for the time being. tonite is a balmy -8 degrees celsius. i spend my evenings on the net. i also stream my tv programs with a site called ovguide. i can watch anything i like. its all free. starbucks allows you 2 hrs free per coffee card that you maintain a $5.00 balance on. but any staples or bestbuy will provide wifi. i keep warm with a propane heater only til i bed down. i won’t risk sleeping with it. i use an air matress on top of a couple of blankets. i then put a blanket or two on top of it. then i put a duvet on top of me. i put some clothes inside my bed to keep them warm for when i get up. but i sleep nude. i use my own breathe to keep it snug and warm. i do wear a toque on my head. i use a little propane burner to cook and i keep a cooler that i fill with snow to save on the cost of ice. i charge my cell and batteries for my lights while i am driving. although my lights are all LED for there battery saving qualities. my propane heater by coleman is awesome. it makes life in the van more homelike. just a note. keep clean and shaven. look after your teeth. wash daily any way possible. it makes it more like the life you are used to. don’t ever wear your shoes in the back where you are living and never get a chill. it will be almost impossible to get warmed up completely. remember why you are sleeping outdoors in your vehicle.. its a choice. not a cercumstance..have fun with it. but don’t tell anyone.. it will come around and bite you in the ass. keep hydrated even in the winter months. it will actually keep you warm..i always make a hot drink of cup-a-soup or hot chocolate just before i go to sleep. it warms me and the van.. life is good.. lol..any questions i can be contacted at dalecasselman@gmail.com…. i am not a bum. i just started my business this year. its called total exterior and i am making out like a bandit. the reason for the van experience. i was living in vancouver, bc. but i am working in calgary, ab. i will head back in another month.
The vehicle I live out of during the colder months when the camp goes through it’s seasonal weather anomalies is a 2001 GMC cube van. It has a ten foot fiberglass box with a counter and a bench. I put my folding cot in the middle and sleep comfortably. My useful necessities are in plastic boxes, I cook at parks, I sleep in a double sleeping bag, have a sawdust bucket for a toilet, a cooler for food . I get ice frozen at a fridge in a local college I attend, and take showers at friends homes. It’s a tough life and sometimes you have to be patient about many challenges but it is a good way to keep expenses low or not have to work as many hours. I was able to pay my land off in eight years. Sometimes I wish I had a house but then remember the stress I was under always having to get the mortgage money up. Someday I will build my little cabin, have already experimented building natural buildings like small domes, underground root cellars, greenhouses. Now the financial stress has been reduced . I don’t get SSI or other income, but because of this lifestyle I can work less than part time while going to school full-time. My life without stress from the monetary needs is more stable and relaxed. If well-planned out, it could almost be very satisfying, unlike other lifestyles. And I am growing an edible perrenniel garden at the camp, picking my own apples, plums, hazelnuts, vegetables, every year. It’s been a rugged lifestyle but it shows what you are made of. (BTW, I’m a woman in my late fifties, it has kept me very fit. I can outwalk, out hike, out roller blade people half my age).
Power Inverter: This is a device you can plug into your cigarette lighter and charge your laptop, cell phone, or any other electronic device as long it is a small enough wattage. It costs about $20 at many stores. Be careful what you charge. Some things will kill the battery if you charge it too long. Try to charge things while driving when possible because it doesn’t use the battery. The one I had had was 100 watts, which means anything you charge has to generally generate less electricity than that.
What’s also key here is power. Especially if things like lighting and internet access are important to you. A DC-to-AC inverter, for instance, can provide electricity to larger devices such as laptops, refrigerators, and the like while driving. However, if you’re planning on parking it for a few days, a robust power bank is the way to go. Here, you can pair and keep these charged through any number of solar panels that can attach to the roof of the vehicle. It reduces the wear and tear on your car battery and alternator, as well as prevent the unthinkable from happening: a dead car battery in the middle of nowhere.
Big Sur, the actual entirety of Big Sur, is stunning. Bordered to the east by the Santa Lucia Mountains and the west by the Pacific Ocean, it’s traversed by narrow, 2-lane highway, known for winding turns, seaside cliffs and views of the often misty coastline. It’s possible (barely) to whip around the winding turns, but who would want to? I enjoyed the slow ride (probably only accelerating faster than thirty-five miles per hour a few times throughout the entire ride through Big Sur.
I had dark tint on my windows some of the time I slept in my car. If you don’t, you can put dark towels up in front of all windows. You have to be conspicuous though and make sure no one is around when you put them up. Just go to your spot, park, shut the car off and set the towels up. That is what I did. Make sure you choose the same place for sleeping every night. It makes things a lot easier. Don’t tell anyone where you sleep.
Living out of a truck may defy the status quo for Cochrane, but in some ways it provides the same security as a traditional home—albeit with a mobile spin. Lizzy VanPatten, a climber who spent the past summer guiding at Smith Rock, makes the case: “I can live wherever I want within driving distance without having to first worry about finding a new home, moving, and getting a job. For me, it’s as simple as picking a place on a map, fueling up, and driving. It makes trying new things a whole lot less intimidating knowing that I can always leave if it doesn’t work out.” Feeling the tug of that sort of freedom? Here’s how to go about it.
I responded to this idea several comments above, please read that entire response with other valuable information. Pet warmers do not heat on 6 watts. That is stated as the continual wattage consumption on pet warmers because they have a device on them, that stays on continually, that you cannot turn off. Since you cannot turn it off, it has to state upfront that the continual wattage use is 6 watts. That device is always on so it can detect when a pet has walked on it and stays there. When it detects weight staying on it, then it turns the heat on.

That is very similiar to the situation I am in. I got laid off because of the bad economy. I am recieving unemployment benefits but they are not enough to pay for both an apartment and a car – a car that I am still financing. I could not even consider giving up the car because that would have put a repo on my record and ruined my credit. A car will also help to make find a new job easier.


I womder if it is ok to park at a travel stop all the time? I know that people that live in their vehicles do not stay in the same place due to not wanting a run in with the cops. But I wonder if it would ok to stay at a Loves travel station every night to sleep. I am about to live in my vehicle in less then 2 weeks. I looked around and found a Loves in a safe area. I really do not want to be going from place to place to sleep. But I will if that is what I have to do to survive. Any advice.

I am concerned that you might be paying for your debts with your kidneys (literally). Or worse. Having a bathroom and cooking facilities is important for your health. You could eat better and cheaper cooking at home than feeding off gas station ramen. And a bathroom helps prevent and cure illness–I lived for some time without running water, so I know.
I ended up starting from the Los Angeles area – so not quite at the Mexican border. It seemed every fifteen miles I was pulling onto the shoulder to take in the view. The ride was more picturesque than I imagined and I hadn’t even reached Big Sur yet. When I finally reached a sign that read, “Welcome to Big Sur”, I stopped the car, hopped out and started exploring.
Owning a car is likely one of your largest personal expenses, and while it will always be one of the bigger expenditures on your personal budget, there are many ways to save big money on your car expenses. As with all personal finance, the more cost-saving measures you adopt now, the more money you'll save. While potential savings will vary widely depending on your personal situation, here are some of the best places to start saving money on your car expenses.
hey you sound like you no how to do things being homeless, i ben homeless for the better part of this summer and right now im living in my truck with some bare essentials in the back and a bed frame in the bed but i sleep inside the cabin and no job except scrapping metal and finding money in parking lot anyway i ben sleeping at apts, walmarts, trcuk stops, rest areas, hotels but am running out of places to stay and getting low on money any suggestions? thanks
They aren’t the only ones. Spending on experiences like food, travel, and recreation is up for all consumers, making up more than 20 percent of Americans’ consumption expenses in 2015. (In contrast, the share for spending on household goods and cars was in the single digits.) Baby-boomer parents, downsizing as they enter retirement, find that their grown children are uninterested in inheriting their hoards of Hummels and Thomas Kinkade paintings. The same “live with less” logic has begun to extend beyond stuff to the spaces these older adults occupy.
Another promising method is based on combinatorial antigen recognition with balanced signaling in which nonspecific antigens A and B can both express on tumor cells while normal ones only express one of them.49 The gene-modified T cells can express both a first-generation CAR with CD3ζ as antigen A recognition element and a third-generation CAR with chimeric costimulatory receptors (CCRs) like CD28/CD137 as antigen B recognition element. When the T cells meet the normal cells that express only antigens A or B, the former cells can only provide a low-affinitive activating signals from CD3ζ without costimulatory signals from CCR while the latter cells just provide costimulatory signals without T cells activating signals from CD3ζ, which in any case provide insufficient T cells activating signals and thus protect normal cells. However, if recognize tumor cells that express both antigen A and B, the activating signals from A and the costimulatory signals from B can work together to active T cells and therefore release granzyme and perforin to kill the tumor cells. The key point of this method is the diversity of affinity in which CD3ζ holds a lower affinity with its target antigen while CCR holds a high affinity, only when the CD3ζ and CCR are binding together with their target antigens can the T cells be activated.
Exploring the molecular basis for decreased SREBP-1c expression, we found that CAR induces the phase II enzyme SULT2B1b, which sulfates and inactivates oxysterol agonists for LXR, a well known activator of SREBP-1c expression. The suppressive effect of CAR activation on lipogenic gene expression is substantially impaired in SULT2B1b knockout mice. Thus, it is likely that this indirect pathway of LXR ligand inactivation contributes significantly to the decreased SREBP-1c expression, and that there are additional mechanisms, including the previously described induction of INSIG-1 as primary CAR target (17).
I worked for a week with a man who has a 5 bedroom house in Cardiff Bay, he decided to rent it out. He lives very happily in a converted van, I'm not talking a really nice thing either, an old van that basically has a double bed, small cooking area and sink and a rack of various cooking materials and spices. He has a gym membership where he showers daily and uses a launderette to do his laundry.
e. A folding, hanging shower stall and a shower bag with nozzle makes for a hot shower even in freezing cold, and one can get dry and re-dressed before even feeling cold. For a floor, a baby inflatable 1-ring mini pool makes a perfect showerpan floor and warmly cleans your feet as you shower. Otherwise, use something else to keep your feet off the ground.
Thanks for all the good information. I don’t think you came off as a privileged person suggesting anything other than how to live in your car and save money. I’m not getting any younger, my kids are all grown and gone now, I’m retired and if I ever want to go any place and see anything, this sounds like the best way to do it before the nursing home. Lol! Maybe I’ll get some tattoos along the way and blog about it. Thanks for the information!
If your cats were indoor/outdoor cats, you can probably keep them with you. However, if they were always indoor cats, and went out very little, I don’t know if you can keep them. If there were indoor/outdoor cats, park near a field that has trees and bushes. I give much detailed information in the other comments I have posted on this website about keeping cats. Just look for JCasil or cat.
There are three requirements that all nations must meet if they are to establish and maintain their freedom and self-determination in the Greater Community. This holds true for both nations living in highly populated regions of space, such as is the case of your world, and for nations that live in remote regions, even in uncharted territories where contact with other races can be very rare and very hazardous. The world has been given to humanity as its world of origin, as its place of residence and as a splendid environment in which humanity could evolve and build its civilizations… Read more »

Suvs and trucks cost more to operate and own. The foamboard that you get for a few bucks in the school supplies section works very well.. it must be BLACK. It just pops in and out and when you’re driving just throw them on the package tray.. they don’t even take up space. Nobody realizes im in the car except the police who have noticed my pattern… I know people are unaware because I’ve gotten handle checkers and people coming up to the car to “Rescue” my dog, thinking he was in here by himself (he likes to sleep on the package tray, they see him in the rear window). To be safe parking just never park somewhere where you could be blocked in and not be able to get away, like the parking spaces on the edges of lots against the curb. Someone could just pull in front of you and then you can’t drive off and escape.
Sometimes you come to rely on a place, and it really is not yours – you don’t own it, but you come to rely on it. And you can get attitudes yourself against people, that is the public. and that can get you into trouble: have an attitude with the public and someone will call the cops on you or worse, complain to the management or owners, who then call the cops and ask you to leave and you are pissed off already. Try not to agitate with the public or the people who notice you, whoever they are, customers or workers. If you feel that agitation coming on, leave ASAP and stay away for a few days.
Only two of my friends know about my situation. I've worked hard to keep it hidden from coworkers and family members. They will only worry about me, and they can't give me the help that I really need. I have blogged about my experience anonymously. I'm bracing myself for the negative backlash from readers — “You're stupid. That's dangerous!” Still, I am excited to be on this temporary journey and just thankful that I have the independence, the mental fortitude, the creativity, and the good health to make this happen for myself. I am $10,000 richer so far in less than one year!
You’d be amazed by how much stuff you can actually lose in such a small space as a truck bed or the trunk of a car. Some of the things we lost in our truck, a Toyota Tacoma, included shoes, phones, hats, and even the keys to said truck once or twice. Past not wanting to lose anything, having a place for everything and keeping everything in its place is a good idea in general. Life on the road is unpredictable enough, so anything you can do to make things easier is a must. For example, if you know exactly where your cooking utensils are, it can help you get your cooking done much more quickly when you need to get back on the road or you’re losing daylight.
I suggest you explore the rest of this site for advice and tips for living in a car or van. I've lived in cars for extended periods, and have lived in a van (well a small bus) now for several years. I've picked up tips that I have learned the hard way, and that I have picked up from others. I hope that some of them will make your life living in a car an easier one.
I used to be a traveler back in 1993 traveling all around England and Scotland with a convoy of new age traveler, all of us sleeping in old ambulances, army trucks, vans, box vans etc. we even had a fire engine with us. Then the government jumped all over us a banned us living together and introduced laws to disband us. It was the most fun time in my life and met great characters I can never forget. I’m might leave my flat and live in my 16′ cabin cruiser on the River Thames and may even buy a van in the future. I plan to live in my vehicles in England in the summer and save up and in October to to Kerala in southern India and spend the winter out there cheap and come back to England in April and do it all over again. Saving up for a river side retirement home in Kerala. I am forty this January so I have around 25 years to get the money together. Good luck and god bless to all those great people here living alternative homesteads. May you be blessed with what you need. Rex.
Liver SREBP-1c is regulated by insulin through liver X receptor (LXR) signaling (18, 19). Cholesterol sulfotransferase 2B1b (SULT2B1b) attenuates LXR signaling by sulfating and inactivating oxysterol agonist ligands (20). Sulfotransferases, like other phase II drug metabolism enzymes, are potential CAR targets (7, 21), and CAR activation does induce SULT2B1b expression (Fig. 5A). We used SULT2B1b null mice to test the relevance of this induction for the effect of TC on SREBP-1c expression. As expected, CAR induction of Cyp2B10 expression was unaltered in the SULT2B1b null mice, and repression of PEPCK expression was also unaffected (Fig. S7A). TC repression of SREBP-1c expression was lost in the Sult2B1b knockout mice (Fig. 5B), while the repression of SCD-1 expression was attenuated. Expression of the LXR target genes ABCG5 and ABCG8 was also suppressed by CAR activation (Fig. S7B), confirming the suppression of LXR signaling. These results indicate that this indirect pathway of LXR ligand inactivation plays an important role in CAR suppression of lipogenesis, although other mechanisms are likely to contribute.
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