Jumper cables: Sometimes for a couple different reasons, I found that my car battery died and I needed a jump. Most likely because I left the lights on or I charged my electronics too long without driving. It was a pain standing in front of a store asking people if they had jumper cables. I eventually got some jumper cables so when my car battery died, all I had to do was ask anyone who had a car around me if they could give me a jump rather than also having to ask them if they had jumper cables too.
For those six months I was able to house sit for a little bit, but still preferred sleeping in my van, and then was parked in my parents driveway for some of it. I was between where I wanted to go and jobs, but knew I always had my safe place to sleep and the necessities with me. I also parked on BLM land, and some parking lots overnight. The only expenses for that time was gas, insurance, cell phone, and food, it was awesome. I now have a house, but still refuse to give up my vehicle that can be converted in case I ever need it, it's my security blanket, if you will.
n. Lighting: candles have come into disfavor as a safety hazard. Presuming you are an adult, use your best judgment. FOr the past 20 yrs I have had a hanging candle lantern with an added rear reflector that I adore. It adds safety and light…The melted wax is added to coiled cardboard in pop cans for quick fire starters. Energy efficient LED lights are an excellent and inexpensive option to candles. For people choosing to park in a Walmart or other lot, you will generally be trying to KEEP OUT the light at night.
I lost my job out in Denver about 4 months back then I spent the rest of my saving on a move to Idaho for another job. Which I was laid off from after 6 weeks so after two months without work I’m pretty much broke. I just sold/pawned/gave away most of my possessions and in 2 weeks I’ll be moving into my ’95 Subaru wagon and driving to Portland to look for work I’ve got heard that the shelters there offer free 24 hr showers and a place to send/receive mail. I’ll admit to being a little anxious about this decision because if my car gets impounded for vagrancy I’m on the street but I’m also somewhat excited as I’ll be living on my own terms with more flexibility than I’ve every really had. I don’t think I have a romanticized view on doing this I’m sure some days I’ll get damn sick of my car but think it’ll force me to be more active than I’ve been in the past. Anyway this is a great thread I’m glad to have found it lots of great pointers and I look forward (sort of) to joining my fellow vehicle dwellers in PDX
I’ve started living in my Toyota Prius for about 3 weeks now. I’m trying to pay off debts and possibly save to put a down payment on s house. So far I’ve had no problems. I work about 50 hours a week as a caregiver so I have access to a bathroom and can wash up there, I also have a gym membership. This was my choice so I can try to get ahead financially, so I try to remind myself every day that I can do this. “Your future is composed of nows”
Wasteful driving habits can double your fuel consumption. Develop gas-saving habits, such as: (1) always accelerate gently; (2) watch traffic ahead of you so you can anticipate slow-downs and avoid stops; (3) coast up to traffic jams by lifting your foot off the gas pedal instead of approaching at full speed and slamming on the brakes. It takes 20% more gas to accelerate to normal speed from a full stop than it does from four or five miles per hour; (4) don't drive too fast or too slow. It takes 20% to 30% more gas to drive at 70 mph than 50 mph; (5) maintain a steady speed on the highway. Avoid getting stuck behind slow cars where you have to slow down to their pace and then speed up to pass. Potential Money Savings: $390/yr.
Private sites, of which there are around 7,000 in the UK, might be a safe bet. These tend to be holiday sites, or they are owned by travellers themselves. They have mod-cons, such as washing and cooking facilities, but they usually don’t want travellers or gypsies as patrons, even those living in a Ferrari, so they may cap the number of nights you can stay or refuse you entry.
Hi jeffmitchum, I would call the Walmarts in the area you want to stay and ask if they allow overnight parking for RV’s. Some do, some don’t. If they do, they don’t mind if you park your car overnight either. I also recommend you try sleeping in your car before you make your move so you can know what you need to do to make it comfortable. I have a hatchback that I lower and a few foam mattresses to make it feel better.
I am sorry about your friend and her boyfriend. I worked nights and you really need a quiet place to sleep in the day. How about renting a storage space near your job, and sleep there in the day. Make it a place for your car and your stuff. Get there as soon as you get off from work, and you can sleep in your own bed, that you will have there. Rent a cheap membership to the Y, and you can clean up there, and you can eat at the soup kitchens. Save as much money as you can, and then get your own studio apartment, near your job. You are working night, so you will not have to be there at night. (storage place) Use your friend’s address, or any friend. The very best to you, and to all, who are trying to make it, while the rich stomp on us daily!
Not being tied down by rent while living out of a vehicle allows for near-infinite mobility. For Reed Rombough, the 26-year-old who runs Nomad Construction, a contracting business that’s as mobile as it sounds, moving into a 2003 Dodge Sprinter finally gave him the freedom to accomplish many of his travel goals. Last year alone, he spent three months climbing in Patagonia and another three months trekking in Nepal and climbing in Thailand. (He also climbed in 14 different states between those trips.) For Rombough, work and life are closely tied together: wherever he parks his van, he picks up contracting jobs; sometimes it’s the other way around. The former allows him to work wherever he wants to be; the latter allows him to explore places he might not otherwise see.
heating pads, two or three, that people use for muscle aches. they only draw out 50 watts each. you can get a 100 watt inverter to plug into your cigareet lighter. but usually these heat pads are designed with an automatic shut off after an hour. so try to find ones that don’t. ask the pharmacist. go online. inverter is about $20 and a heat pad about $13. try to find a salvage store or liquidation/outlet store.

Now two methods have been worked out. The first method is the utilization of inhibitory CAR (iCAR) which relied on two different antigens.48 Nonspecific antigen A can be expressed on either tumor cells or normal cells while antigen B can only expressed on normal ones. The gene-modified T cells can both express a CAR as antigen A recognition element and an iCAR as antigen B recognition element. Normal cells expressing both antigen A and B, when they are recognized by CAR-T cells, iCAR will produce an inhibitive signals through Programmed Death-1 or Cytotoxic T Lymphocyte-associated Antigen-4 to attenuate the T cell active signals come from CAR, which prevent T cells from activating and attacking normal cells and thereby avoid off target attacks. Nevertheless, when the modified T cells meet tumor cells that only express antigen A, the signals produced by CAR will activate T cells and therefore release granzyme and perforin to kill the tumor cells.


Research shows that the 18-to-35 cohort continues to rent at higher rates than previous generations: 74 percent lived in a rental property in 2016, compared to 62 percent of Gen Xers in 2000, according to the Pew Research Center. And while the Millennial desire to not buy homes tends to be overstated – studies suggest many want to own, but often can’t afford to – they do prioritize experiences over stuff.

I live in Florida. I’m 49, married and have two kids who are about to start college. My wife and I met in college and have been working in corporate America since we were teenagers. We are doing very well and life has been simple but good to us. However, I’m terribly overweight and suffering from anxiety and general lack of interest. Physiologically, I’m getting near my breaking point. Lately, all I do is dream about running away from everyone and everything. Even though our net worth is very high, I dream of buying a VW Budd-e EV Van and living out of it with just a cell phone, laptop and 3 pairs of jeans. I imagine I could shower in 24 hour fitness and eat out every day. I could just drive up and down the east coast, parking at the beach or charging stations near points of interest. Just to test the theory, I’ve take short hall-pass trips for 2 – 3 day and done it. It felt amazing and liberating to not to have a schedule to be that free. I never felt unsafe or uncertain like I do in my regular life.
I agree with Eric. But it’s not only women you have to worry about. It’s men too. People have an image of someone that doesn’t have a home as a dirty, tangled hair alcoholic drug addict panhandling in the street. I am far from that stereotype. I’m an attractive girl fit girl. I work out 6 days a week. I eat healthy, dress nice and have a new car. I am going to start living out of my car as of February 2009 so I can pay off the wealthy bankers that just got a bail out for my $30,000.00 debt plus all the extra finance charges and late fees that I have accumulated in one year for lack of payment. Times have changed and I am not making what I use to make so the only alternative I have is to live out of my car so that I can pay them off and be debt free.
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This next stage was tremendously difficult. I was taking a ton of medication, going for frequent blood tests and still feeling extremely lethargic, sleeping most of my days away. The side effects of some the meds were terrible. I seemed to struggle with either sleepless nights or vivid nightmares. I also felt the need to eat anything and everything day or night (I even stopped wearing my retainer to bed because I was scared I was going to eat it in my sleep!).
After college I lived in my van for a year — partly due to finances but mostly to step back and get some perspective. Roamed the country. One of the best things I ever did. It’s just as easy to break into an apartment or house as it is a car, so the ‘safety’ issue is something of a red herring. Bravo for questioning the ‘received wisdom of society’ that you have to live a certain way (and spend a certain minimum amount). Our options are largely bounded by our imaginations.

No place will really let you setup camp in their parking lot. Truck stops, Walmarts, etc. are lenient b/c truck drivers & RV’ers buys a ton of gas. And they know that they will leave after a night’s sleep or so. But it’s not for vehicle dwellers. If they allow it, people can sue them should something bad happens as their parking lots are not zoned nor suitable for dwelling. You need to blend in and be stealthy. Many people get a cargo van for this purpose and are able to park like they’re there working. Apartment complex parking lots are another good place to park. But you cannot stay more than 2 nights at the same place. You need to scope out a new place just about every night. Anyone would immediately notice a stranger’s car in front or close to their house, let alone one that’s parked there night after night. And always stay away from playgrounds, parks with lots of kids, school zones, etc…as a van hanging out too long will look very suspicious. With out of state tags, it makes things even harder. Good luck.
In order to avoid drawing attention to yourself, you’ll need something that can tint or completely cover the windows of your vehicle. At night especially, a vehicle with light inside or a pile of supplies will be conspicuous. And, if civilization is still around, you’ll want thick curtains to block out the light from streetlamps and other sources while you sleep.
If you’re wanting to stay where you are you can search craigslist for rooms/shared and in smaller cities find rooms for around $300. If you stay where you are you could keep your part-time job as well. You could probably find another female roommate easily depending on where you live. Not an ideal situation but better than living in a car. Could try that for several months to at least get through the winter and plot out something new. Times are so bad for so many that many people are looking for roommates and will do month to month and don’t care as long as you don’t have an eviction.
I work overnight at a Walmart in North Carolina and they have signs stating “No Overnight Truck or RV Parking”. Even though it is chilly in mid-January, we get several trucks parking every night, as well as smaller RVs and other vans and trucks. The managers couldn’t care less, and the cops that frequent the store ignore all but the most obnoxious behavior.

Suvs and trucks cost more to operate and own. The foamboard that you get for a few bucks in the school supplies section works very well.. it must be BLACK. It just pops in and out and when you’re driving just throw them on the package tray.. they don’t even take up space. Nobody realizes im in the car except the police who have noticed my pattern… I know people are unaware because I’ve gotten handle checkers and people coming up to the car to “Rescue” my dog, thinking he was in here by himself (he likes to sleep on the package tray, they see him in the rear window). To be safe parking just never park somewhere where you could be blocked in and not be able to get away, like the parking spaces on the edges of lots against the curb. Someone could just pull in front of you and then you can’t drive off and escape.


This applies to life in general, really, but especially life on the road. When I lived out of a truck, I felt more free than I ever have. Eric and I had virtually no responsibilities aside from keeping gas in the tank and food in our bodies. If we wanted to go to the Grand Canyon, we went. If we wanted to go to the Badlands, we went. If we wanted to bum around for a day and take a few naps, we did. When your entire immediate life is attached to four wheels and an engine, you go wherever you want, do whatever you want, and nobody can tell you not to. You answer exclusively to yourself. Very rarely in regular life (or, at least, what our society sees as regular) do we get this kind of freedom, so while you do have it, run with it. Run as fast and as far as you can until you can’t run anymore, and if you’re still not ready to stop, park in the shade, take a nap, and start running again. Meet all the people, see all the places, do all the things, and take some time whenever you can to appreciate just how lucky you are to live such a beautiful life.


Spare keys container: Having spare keys around are very important while sleeping in your car. You never know when you may need them. I kept a spare key for my car always in my wallet. Also, I went to an automotive store and got 2 containers for about $10 that store keys and have a magnetic cylinder on the back so you can connect it to any metal at the bottom of your car for when you lose or lock your keys in your car. Make sure to put it where no one can see it. Make sure no one knows it is there. Only you.
And speaking of sleep, Odom's chapter on getting deep sleep offers valuable advice for finding the best spots (if you have a white van like his, for instance, parking next to a row of delivery vans at Sears will make you camouflaged forever), and best tactics to avoid nighttime interruptions. If you're seriously considering moving into your wheels, this book might be a valuable packing item, especially because Odom offers his customers his lifetime guidance via email should they have further questions on the road.
Starting this Tuesday after Memorial, I will be driving out to California to begin a new chapter in my life, titled “My adventure to freedom and a newer me”. I will be living out of my car for the next 4 months at the least. This is not a must but a choice which is why I feel good about the title. And, this will not be a first experience for me since I have lived out of my car, different car, in my earlier years. For a almost a year I lived out of a Pontiac Grand Prix. And, In this situation I had to do it. Today I choose to go back to that life style cause I want to rid myself of the waste and the lack of drive towards self achievement. What do I mean? I meant the monotony of my everyday routine of sleep, 45 minute drive to work, 8hrs of yes sir no sir, 45 minute drive back home, eat, watch tv, contemplate going to the gym but instead, falling asleep on the sofa.(what a waste of gym membership), and then do it all over again. Sure, I had a very good paying job. Between my wife and I, we made 97,000 last year. We own a 4 bedroom house in an affluent suburbs. We have 2 grown children with their own careers and one married. I had established a decent savings and few retirement portfolios. My friend envied me because of my salary. I have it good in the eyes of most people, right? Wrong!!!!!!!!!!!!!!!!!!!!!! I was in a cage feeling trapped, and the only way out was to wait for death. I felt something missing! Something that I will regret at my death bed if I continue this path of life. Most people of my age go through midlife crisis and do things to deal with it best they know how. Hell, my neighbor bought a Harley and huge RV. I wish I had his RV, LOL!!!

For the summers of 2015 and 2016, I decided to start living in a car to save money as I took the slow ride up the west coast of the United States along the infamous Highway 1. I started from Mexico and drove north in one of the best cars to live in; I finally turned back after a few weeks exploring National Parks in Canada which should be on everyone’s car camping list of places to stay.
Lots of people say they are CHRIST ian and are not. lots and lots and lots. good and evil people. It is not a fact that she is a CHRISTian. you certainly don’t know either. Do not be hateful of people living in that situation or on less because CHRIST and the APOSTLES lived on less and were hated. I don’t know what she is or what you are. you sure assume that you know me and her also.

Where will you be parking this car? Are there local ordinances that limit overnight parking? Is there a local ordinance prohibiting sleeping in public, or camping in the city limits? Or idling your vehicle for a period of time? Will there be children living in the car? If so, then per state law, they must have running water. Where are you going to get that? Running hot and cold water, in your car.

Why is it the aunt & uncle’s responsibility to fund their nephew’s lifestyle? If he had been truly doing his best to get his financial life in order, then I would say yes, they could help him out. It sounds like he wasn’t making wise financial choices at that point in time. And he was costing them money that they could be getting by renting the room to a non-family member. I don’t feel like they did anything wrong.
I work nights and spend the days at a Korean Spa for about $12.00 per day. They usually have sleep rooms, fresh towels, and a way to pamper myself when I wake up. Unfortunately my home is several states away and is rented out at a loss…. but I still have a mortgage payment since the market is so bad. At my age I have to hold onto something. I never thought I would be homeless.
I was doing this so I could save money and get ahead in life. You have to sacrifice in life if you want to get ahead in life. That’s what I have learned. Especially in this economy today, you never know when hardship may hit and having these survival skills in your pocket may just save your life one day when you experience hardship. I did have a job and no one would have ever guessed I slept in my car and that is how it should be. More attention is worse when living in one’s car.
So other than your student loans, which will be forgiven if you stayed employed for 5 consecutive years, you are debt free? Is it your goal to save up enough money to pay cash for a replacement car? Once that’s done, then what? Will you continue to live on your NEW car? Or will you feel like you can go & rent a room or apt somewhere? While you told us where you’ve been & where you are now financially, I’d like to know where you want to go in the future, past saving for a car. While I applaud your fortitude go make drastic changes, I can also see that you could have the tendency to continue to live in your car as some others have already commented. You have to do what’s best for you, but you also have to think about how far you’re going to hit your goals (whatever those are).

I found this website by accident and then thought of a solution for many having financial problems these days. When my husband and I retired we wanted to camp at leisure without a time line or reservations for 2 months out west to recapture our youth. I can no longer camp in a tent since I have back and knee problems and cannot get up and down easily. We had a van. We took out the back seats. I bought 12 flip top crates from Costco and fit them in the space. I bought no-see-um screening from Campmor (It’s very cheap). I cut the screening to cover the side doors and back door leaving enough for hems. I bought hundreds of round magnets at Rag Shop and sewed them into the hems about 3 inches apart. This was how I attached the screening to the van. We had an old 4 inch thick foam mattress I cut to cover the crates in which we kept everything including our photographic equipment. I made 2 sheets to fit the foam mattress. We used no suitcases. We brought 2 pillows each and 2 sleeping bags. I put up heavy cord between the garment hangers to hold the car rechargeable Coleman lantern I used at night for reading. My husband bought pvc pipe which he attached to the roof rack on evenings where rain was forecast. If we had rain we put it over the extended pipes to keep the rain from coming in the doorways. We slept comfortably in Yellowstone down to 19 degrees. We had a blast. We only spent extra money on campgrounds at half rate using the golden senior pass. I am sure these suggestions would help someone to survive a period of time to pay off debt and get a new start. We came home to our residence renewed with the thrill of how little our trip cost us. We will be doing it again soon and save money to go on more expensive journeys to other places in the world ala elder hostel.
Living out of a truck may defy the status quo for Cochrane, but in some ways it provides the same security as a traditional home—albeit with a mobile spin. Lizzy VanPatten, a climber who spent the past summer guiding at Smith Rock, makes the case: “I can live wherever I want within driving distance without having to first worry about finding a new home, moving, and getting a job. For me, it’s as simple as picking a place on a map, fueling up, and driving. It makes trying new things a whole lot less intimidating knowing that I can always leave if it doesn’t work out.” Feeling the tug of that sort of freedom? Here’s how to go about it.
I would not advise getting batteries from a junk yard. waste of money, and they could be leaking or damaged. they certainly wont hold a lot of power and go bad fast. Batteries that have been dormat for a long time are NEVER good. not even new ones. Autozone rotates their NEW batteries every six months. if the battery was made (not shipped but made) more than six months ago, they take it off the shelf. They will not sell it, and they give only a 1 year replacement warranty on NEW batteries.
You sure are right about people being judgmental and assuming we don’t work or pay taxes. When I tried to get a library card the librarian recognized my proof of address as the post office and said without a residential address I am not a citizen (I live, work and go to school in this city) and cannot have a library card because “The library is funded by the taxpayers.” I pay hefty self-employment taxes and taxes every time I buy something! Then, she went on to say I could have the non-resident card for $200/year. … I just went to another library in the system and they gave me a card. If you don’t pay property taxes you’re considered a second class citizen.
GUYS just spent half an hour typing about storage units, unfortunately it all got deleted sorry i have some good info for the combination of the two things, storage unit, and car homelesness, whether we are homeless or not this is how are seen. let me just say that if u are paying for a storage unit then my suggestion would be to sleep in your vehicle there you are already paying rent. you have every right to be there, and alot of people run business’s out of them. keep your head up and never loose your sense of superiority, because we are better than the people stuck in the rat race, they just dont know it and we care to much wat they think about us

a. Organize, organize, organize! Have a place for everything and keep everything in its place. In addition to your sleeping items, you will need a carry-on style suitcase for clothes, a laundry bag for clothes pending a trip to the laundry (with air freshener), a “chuck box” (your car camping kitchen supplies), water storage container and a cup/water bottle, a tool box, a briefcase organizer for paperwork, a box with your camping supplies, a toiletry case (with towel, washcloths, shampoo, soap/shower gel, hairbrush, other hygiene supplies), flashlight/LED lamp and candles.
Those survival blankets? the ones that look like aluminium foil? they are good. It takes a lot to rip them, they are cheap and take up hardly any room in a pack. If you are in a tent or a hammock wrap yourself in one of those emergency blankets and then get into your sleeping bag. Socks, hat, gloves. If you choose a hammock, get one without a space bar and made of parachute nylon which folds up small, Ticket to the Moon makes a good one, buy a double size and sleep in it diagonally, that way your body will be flat and not like a banana. Hennessey is good too. Maybe tape another foil blanket to the surface of your hammock underneath. If you put the foil between hammock and sleeping bag it gets all scrunched up. good luck. If your pool showers have cubicles you can shave and clean teeth in the shower where no one will see.
I have got this down to a science now. I got a storage unit to put my stuff in. I put my laptop on the floor of the passenger seat. I have a small sedan. At bedtime I put two fluffy pillows ontop of the laptop case. I recline the seat back and sleep in it upsidedown! My legs are a bit elevated but I used to sleep this way anyway to rest my sore legs. I do go to sleep early like around 8pm because I work at 7AM. I find the darkness to be my friend. At 8pm I can move my bedding from the trunk to the back seat I have tinted windows so know one can see whats in there. Also I can get my clothes together for the next day work uniform and clothes for after work. these I also keep in three small boxes in the trunk. One for clean undies, sock and bras. the second is for work clothes and the third is for regular clothes. My trunk is pretty full. I wake at around 4AM to got to the gym and workout then shower and off to work.
Adding wires does not modify the vehicle. like adding a superior stereo system is not considered modifying the vehicle. WalMart itself sells different kinds of vehicle inverters and wires and stereo systems. They want you to buy those things when you are having your car serviced there. If they say you have modified your car, you can walk them through the store and show them the inverters that they sell or go online with your laptop and SHOW them the many inverters that WalMart sells.
I lived in my car in Silicon Valley for three months. It was pretty bittersweet. I would recommend it. There are obviously some minuses to living in a car, but especially if you’re passionate about minimalism or alternative lifestyles, it’s very doable. I even found that you can turn living in a car into a productivity hack, and with a monthly burn rate under $300, you could make very little last a very long time.

Unfortunately, my nomad adventure only lasted 2 years. the Jubilee-mobile broke down – twice. Good thing, I needed something to force me to stay put. I now have a grandson that nothing short of an act of God could separate me from. If one day my daughter doesn’t need me…well, I already have another van and plenty of plans to remodel. Better yet, I’m hoping when he’s older she’ll let him come with me.


I’m a 63-year-old woman about to be homeless for the first time in my life. After living a hard-knock life (I’ll spare the details), I had a decent job with a decent wage, and was finally able to buy a little house, which I thought would be the beginning of a peaceful ease into retirement for me. Four months after I bought the house, I lost my job. I got unemployment for a while, but that ran out. I’ve been unable to find a decent job since then (it’s been 2 years now – I’m convinced that age discrimination is alive and well in the good ol’ USA), I was forced to start taking my Social Security as soon as I was eligible for it, because I had absolutely no other means of income. I also started the process of applying for a loan modification from the bank that has my mortgage (BofA), so that I could stay in my home, and continue to make my payments, but hopefully at a reduced interest rate, and lower payments that would be affordable on my SS income. After a year of endless hassle and fighting, not to mention tears and near mental breakdowns, I was denied an affordable loan modification, and I’m now facing a short sale. I’ve sold most of my furniture and all of my grandmother’s silver, as well as most of my own good jewelry, and I’ve rented a small storage unit for the few belongings that I feel I want to keep, and am now just waiting for “permission” to vacate my now-empty house. I do have a small SUV which is finally paid for, but I’m thinking about selling it and trying to buy a small RV to live in. My thought process is that I think I could afford moderate payments on a used RV, but I sure as heck know I can’t afford rent on a small apartment somewhere – unless anyone knows where I can get a decent (no ghetto or crack house, please) apartment for about $300 a month? My SS is only about $1,000 a month. Period. People I talk to think I’m crazy, but I seriously don’t see any alternative. No one knows how seriously depressing this is for me, or how close to suicide I have been over the past year. The only thing that keeps me going is my little dog, who I will never abandon.
Keeping your car tidy and your stuff neatly stowed away serves two purposes. First, it makes your life in your car far more civilized and less like dwelling in a mouse nest. Second, your car will appear to outsiders more like a regular car, and you will avoid attracting unwanted attention with obvious signs of homelessness. Small plastic totes may be your best friend in the quest for car organization. Choose a size that you can stack six of easily in the available storage space area of your vehicle. A variety of colors will make finding your stuff more intuitive, though labels are also essential (use a permanent marker and big block letters). Sample labels:
I was looking at the VW Campers but where can I find one in good shape? I too will be living in my car in about 2 days….its a voluntary thing I want to pay off my credit. With paying rent, a little on back bills, car payment, insurance and classes by the time my I get my check on Friday its gone friday evening…this doesnt allow for social things…so the debate is eiather rent or school at this point…Nothing ever outweighs my education!!!!
I too live in southern california, work and am a student. I rent a master bedroom in the suburbs. I am considering living out of my honda crv because it has fold-down rear seats and tinted windows. I could save $550/per month, not to mention gas. But, I am a girl and I guess that could be particularly more risky as far as safety. I have mentioned to my older sister and she said “no way!” and my ex-boyfriend initially said no way, but after hearing me out, didnt really seem to have any other good arguments left. :oP
This information is intended to support, not replace, discussion with your doctor or healthcare professionals. The authors of these consumer health information handouts have made a considerable effort to ensure the information is accurate, up to date and easy to understand. The Royal Children's Hospital, Melbourne accepts no responsibility for any inaccuracies, information perceived as misleading, or the success of any treatment regimen detailed in these handouts. Information contained in the handouts is updated regularly and therefore you should always check you are referring to the most recent version of the handout. The onus is on you, the user, to ensure that you have downloaded the most up-to-date version of a consumer health information handout.
MUC1 is a transmembrane glycoprotein, whose overexpression has been widely reported in several malignant cancers, including liver cancer.28 Therefore, MUC1 becomes an ideal target of immunotherapy for liver cancer, due to its overexpression. For example, Ma et al. have engineered first- and third-generation CAR-T against MUC1 to treat MUC1 overexpressed liver cancer. Their results reveal its potential of serving as TAA for liver cancer.29
Liver SREBP-1c is regulated by insulin through liver X receptor (LXR) signaling (18, 19). Cholesterol sulfotransferase 2B1b (SULT2B1b) attenuates LXR signaling by sulfating and inactivating oxysterol agonist ligands (20). Sulfotransferases, like other phase II drug metabolism enzymes, are potential CAR targets (7, 21), and CAR activation does induce SULT2B1b expression (Fig. 5A). We used SULT2B1b null mice to test the relevance of this induction for the effect of TC on SREBP-1c expression. As expected, CAR induction of Cyp2B10 expression was unaltered in the SULT2B1b null mice, and repression of PEPCK expression was also unaffected (Fig. S7A). TC repression of SREBP-1c expression was lost in the Sult2B1b knockout mice (Fig. 5B), while the repression of SCD-1 expression was attenuated. Expression of the LXR target genes ABCG5 and ABCG8 was also suppressed by CAR activation (Fig. S7B), confirming the suppression of LXR signaling. These results indicate that this indirect pathway of LXR ligand inactivation plays an important role in CAR suppression of lipogenesis, although other mechanisms are likely to contribute.
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